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Part:BBa_M10020:Design

Designed by: Nikhil Sharma   Group: Berkeley BioE140L - S09   (2009-05-10)


{˂TraT˃} C-terminal Display Protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 103
    Illegal PstI site found at 142
    Illegal PstI site found at 401
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 103
    Illegal PstI site found at 142
    Illegal PstI site found at 401
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 103
    Illegal PstI site found at 142
    Illegal PstI site found at 401
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 103
    Illegal PstI site found at 142
    Illegal PstI site found at 401
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 579
    Illegal BsaI.rc site found at 61


Design Notes

-Had to remove the prepro sequence


Source

Construction of {<TraT>} part

PCR Ons001F/Ons002R on TraT (700 bp, BamHI/EcoRI) Digest pBca9495CA-Bca1144#5 (BamHI/EcoRI, 3039+910, L) Product is pBca9495CA-M10020 {<TraT>}

Ons001F Forward oligo for TraT ccataGAATTCatgAGATCTtgtggtgcgatgagcacagc Ons002R Reverse oligo for TraT ctgatGGATCCgagaatatttgcgattgatttgg

References